Effects of Laminaria japonica polysaccharides on exercise endurance and oxidative stress in forced swimming mouse model
© Yan and Hao. 2016
Received: 21 September 2015
Accepted: 13 April 2016
Published: 26 April 2016
Polysaccharides are the major active ingredients responsible for the bioactivities of Laminaria japonica. However, the effects of L. japonica polysaccharides (LJP) on exercise endurance and oxidative stress have never been investigated. Therefore, this study was conducted to investigate the effects of LJP on exercise endurance and oxidative stress in a forced swimming mouse model. The animals were divided into four groups, namely the control (C), LJP-75, LJP-150, and LJP-300 groups, which received physiological saline and 75, 150, and 300 mg kg−1 LJP, respectively, by gavage once a day for 28 days. This was followed by a forced swimming test and measurements of various biochemical parameters.
LJP increased swimming time to exhaustion, the liver and muscle glycogen content, and levels of superoxide dismutase, glutathione peroxidase, and catalase in the serum, liver, and muscle, which were accompanied by corresponding decreases in the malondialdehyde (MDA) content of the same tissues. Furthermore, decreases in blood lactic acid and serum myeloperoxidase (MPO) levels were observed.
LJP enhanced exercise endurance and protected mice against exhaustive exercise-induced oxidative stress.
KeywordsLaminaria japonica polysaccharides Forced swimming test Exercise endurance Oxidative stress Mice
The generation of reactive oxygen species (ROS) is a necessary and unavoidable consequence of aerobic metabolism . The enhanced oxygen consumption during exercise leads to an increased flux of oxygen through the mitochondria, and 2–5 % of this oxygen is not completely reduced to water and, therefore, generates ROS . Under normal physiological conditions, cells have adequate defenses against ROS production and enough endogenous enzymatic and nonenzymatic antioxidant reserves [3, 4]. However, during strenuous physical exercise, the rate of ROS generation exceeds that of their removal and oxidative stress occurs . Consequently, accumulated excessive ROS can attack vital biomolecules such as plasma membrane lipids and proteins and, thereby, deteriorate normal cellular functions and further contribute to muscle damage . Specifically, it has been shown that strenuous physical exercise decreases antioxidants levels and increases lipid peroxidation markers in the blood and tissues . Therefore, antioxidant supplementation may protect against exhaustive exercise-induced oxidative stress by forming less active radicals or quenching free radicals and ROS . Many antioxidant bioactive compounds, such as polysaccharides from Radix pseudostellariae [Pseudostellaria heterophylla (Miq.) Pax], polysaccharides from Auricularia auricula, polysaccharides from Cordyceps sinensis mycelium, polysaccharides from Ganoderma lucidum, salidroside, ginsenoside-Rg1, ginsenoside‑Rb1, flavonoid from Citrus limon (L.) Burm. F. as well as polyphenols from Vaccinium corymbosum L., have been reported for their protective effects on exhaustive exercise-induced oxidative stress [4, 9–16].
The brown seaweed Laminaria japonica, is a common seafood consumed in China and numerous other countries and has been documented as a drug in traditional Chinese medicine (TCM) . In the ancient literature, L. japonica has been recorded as an important therapeutic agent for phlegm elimination, detumescence, and weight loss for more than 1000 years . Over the past decades, L. japonica has been the focus of attention of chemists and pharmacologists because of its abundant functional compound content and the associated biological properties. The major active constituents of L. japonica are polysaccharides including alginate, fucoidan, and laminarin . Recent studies have demonstrated that L. japonica polysaccharides (LJP) have a wide range of biological properties including anti-apoptosis, antivirus, anticoagulant, antitumor, antithrombotic, anti-radiation, hypoglycemic, hypolipidemic, and immunostimulatory [20–23]. Furthermore, LJP protected endogenous antioxidant enzymes, inhibited lipid peroxidation, and exhibited high antioxidant activities including the oxygen radical absorbance capacity (ORAC), 2,2ʹ-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and reduced power tests [24, 25], suggesting that LJP might reduce exhaustive exercise-induced oxidative stress. Therefore, the current study aimed to demonstrate the protective effects of LJP against exercise endurance and oxidative stress in a forced swimming mouse model.
Results and discussion
Effects of LJP on swimming time to exhaustion of mice
Effects of LJP on blood lactic acid levels of mice
Effects of LJP on glycogen contents of the liver and muscle of mice
Effects of LJP on superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) levels of mouse serum, liver and muscle
It was previously demonstrated that antioxidant enzymes play a significant role in protecting the body against ROS . The principal antioxidant enzymes include superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT), and they act to reduce ROS . Regular physical exercise has been shown to increase antioxidant enzyme activities in the blood and tissues of humans and animals [31, 32], which can be attributed to a compensatory response to counteract the possible detrimental effects associated with oxidative stress . However, other studies have reported that strenuous exercise causes a dramatic drop in antioxidant enzyme activities in the blood and tissues . Although inconsistent findings have been reported on the level of antioxidant enzymes, it appears that the variation of these enzymes is dependent not only on the type of tissues measured but also on the mode and intensity of exercise .
Effects of LJP on malondialdehyde (MDA) content of mouse serum, liver and muscle
Effects of LJP on serum myeloperoxidase (MPO) levels of mice
This study demonstrated that LJP enhanced the exercise endurance of mice by increasing swimming time to exhaustion and the glycogen contents of the liver and muscle, as well as decreasing BLA levels. Furthermore, LJP exhibited a protective effect against exhaustive exercise-induced oxidative stress by increasing the serum, liver, and muscle levels of SOD, GPx, and CAT, as well as decreasing serum MPO and the MDA contents of the serum, liver, and muscle. The experimental data have shed some light on the clinical therapeutic potential of LJP against exhaustive exercise-induced oxidative stress. However, further study is required to ascertain the detailed underlying mechanisms of these effects.
Laminaria japonica was collected in Zhoushan, Zhejiang, China in September 2013 and the plant material was identified by Professor M. J. Wang (College of Life Sciences, China Jiliang University, Hangzhou, China). The fresh L. japonica samples were immediately washed with water, sun-dried, ground into a fine powder using a mechanical grinder (FZ102, Taisite Instrument Co., Tianjin, China), filtered through a 40-mesh (200-μm) sieve, and then the dried powder was stored at room temperature (20 ± 2 °C) in a desiccator (300 mm, Huaou Industrial Co., Yancheng, China) until further used.
Chemicals and reagents
Purchased commercial diagnostic kits were used for the determination of BLA (Leadman Biochemistry Technology Co., Ltd., Beijing, China); tissue glycogen, SOD, GPx, and CAT (Jiancheng Biotechnology Institute, Nanjing, China); MDA (Biosino Bio-technology and Science Inc., Beijing, China); and MPO (Jianglai Biochemistry Technology Co., Ltd., Shanghai, China). All other chemicals and reagents were of analytical grade purity, and they were purchased from Hangzhou Chemical Reagent Co., Ltd. (Hangzhou, China), and were used without further purification.
Preparation of LJP
The LJP was prepared according to previously published method [21, 45] with minor modifications. Briefly, dried powder sample was defatted with anhydrous ethanol at 60 °C for 3 h with stirring and then mixed with distilled water thrice (1:40, w/v) at 90 °C for 2.5 h. The insoluble residue was separated from the aqueous extract by centrifugation (10,640×g for 15 min). Then, the combined supernatants were concentrated to a quarter of the original volume by evaporation and deproteinated using the Sevag method . The solution was added to anhydrous ethanol to obtain an ethanol concentration of 80 %, kept overnight, and then filtered. The resulting precipitate was dissolved in water followed by the addition of anhydrous ethanol to a final ethanol concentration of 80 % and then filtered twice. The precipitate was washed sequentially with 95 % ethanol, anhydrous ethanol, and acetone and then lyophilized to obtain the final extract of LJP at a yield of 21.37 % (w/w) of the original L. japonica plant material. The dried LJP was dissolved in saline solution just before use.
Adult male Kunming mice (Mus musculus, Km, with weight 20 ± 2 g) were procured from the Experimental Animal Center of Zhejiang Province. All animals were housed under standard environmental conditions (temperature 21 ± 2 °C; humidity 45 ± 5 %; and 12-h light:dark cycle) with free access to a standard pellet diet and water ad libitum. All animal studies were performed according to the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (NIH), as well as the guidelines of the Animal Welfare Act. The experimental protocol was approved (approval number: ZJSR2014‑0113) by the Institutional Animal Care and Use Committee (IACUC) at the Zhejiang Shuren University.
The mice were allowed to acclimatize to the laboratory environment for 1 week prior to the experiments. Then, the animals were assigned randomly to four groups of 12 mice each namely the C, LJP-75, LJP-150, and LJP-300 groups, which were treated with the vehicle (physiological saline) and 75, 150, and 300 mg kg−1 of LJP, respectively, for 28 days. The LJP was dissolved in 1.5 mL of the vehicle, and the C group received the same volume of the vehicle as well. The treatments were administered orally by gavage once a day according to the pretest and dose determined during the active screening.
After the final LJP or vehicle treatment, the mice were allowed to rest for 30 min, and then they were subjected to the forced swimming test using the method described by Li et al. . The apparatus used was an acrylic plastic pool (60, 50, and 50 cm in length, width, and height, respectively) filled with fresh water, which was maintained at 25 ± 0.5 °C at a depth of 40 cm. Each mouse was weighted using a lead wire bundle attached to the tail at 5 % of the body weight. Exhaustion was determined by observing the loss of coordinated movements and failure to return to the surface within 10 s.
Following the forced swimming test, the mice were anaesthetized with absolute ether and the success of the anaesthesia was confirmed by verifying the absence of reflex responses to noxious stimuli. Then, the mice were euthanized by decapitation, blood samples were collected for BLA analysis, and serum was obtained by centrifugation (2000×g, 4 °C, 10 min) for the SOD, GPx, CAT, MPO, and MDA analyses. After blood collection, the liver and gastrocnemius muscle tissues were quickly dissected, washed in ice-cold physiological saline, frozen in liquid nitrogen, and stored at −70 °C for the assays of glycogen, SOD, GPx, CAT, and MDA. The measurements were performed according to the recommended procedures provided by the commercial diagnostic kits.
The data obtained were expressed as mean ± standard deviation (SD). The results were analyzed using a one-way analysis of variance (ANOVA) followed by a post hoc Tukey’s test using the statistical package for the social sciences (SPSS) software (version 15.0, SPSS Inc., Chicago, IL, USA). Values were considered significant when p < 0.05.
Laminaria japonica polysaccharides
blood lactic acid
FY designed and planned the study and drafted the manuscript. HH performed the analyses and collected the test results. Both authors cooperated on the interpretation of the results. Both authors read and approved the final manuscript.
We are grateful to Dr. Lijun Li (Jilin Agricultural University, China) for his revision and comments on the manuscript. This study was funded by a Grant (No. ZGT201124) from the Association of Higher Education of Zhejiang Province, China.
The authors declare that they have no competing interests.
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