Fig. 4

Increased expression of Tbx20 decreased ALP and ColI expression. Representative micrograph of 14 days incubation of Ad-MSCs exposed to LV-Ctrl or LV-Tbx20 with EGFP-positive, ALP and ColI expressions (a–d). Ad-MSCs overexpressed Tbx20 demonstrated two- and threefolds reduction in ALP and ColI expression respectively compared to LV-Ctrl (e, f). ****Shows significant differences (p < 0.0001) between LV-Ctrl and LV-Tbx20. Experiments were performed in triplicate and repeated three times