Fig. 3

Aβ treatment promoted COX-2 expression and PGE2 secretion, and activated NF-κB pathway in SH-SY5Y cells. SH-SY5Y cells was exposed to different concentrations (0, 10, 25, 50 μM) of Aβ for 48 h. a qRT-PCR analysis of COX-2 mRNA expression. b Western blot analysis of COX-2 protein level. c ELISA assay of PGE2 concentration in culture supernatant. d qRT-PCR analysis of mPGES-1 mRNA expression. e ELISA assay of NF-κB protein levels. f Western blot analysis of MCL-1 and cyclin D1 protein levels. *p < 0.05 vs. controls