Fig. 5From: Modulation of RECK levels in Xenopus A6 cells: effects on MT1-MMP, MMP-2 and pERK levelsSolubilization of RECK proteins following PI-PLC treatment. a Twenty-four hours following transfection of RECK constructs, control and transfected A6 cells were treated with PI-PLC for 24 h and cell lysate collected and analyzed by Western blot. b Densitometry quantification demonstrates that the level of RECK proteins bound to the cell is significantly reduced following PI-PLC treatment in both mock-transfected and RECK-transfected cells. β-actin is used as a loading control. Graphed data is based on 3 biological replicates (mean ± SD). Data is analyzed via t-test; *, p ≤ 0.05; **, p ≤ 0.01Back to article page