Fig. 6From: Aberrant methylation-mediated downregulation of lncRNA CCND2 AS1 promotes cell proliferation in cervical cancerSilencing of CCND2 AS1 due to its promoter CpG methylation in cervical cancer. aCCND2 AS1 expression was restored when the HeLa and SiHa cells were treated with 5΄-Aza from 5 μM to 50 μM. b Diagram of the CCND2 AS1 gene promoter with the transcription start site (TSS) indicated. The short black double-headed arrow represents the location of the fragment detected by the MSP and BGS assay. c Methylation status of the CCND2 AS1 promoter was detected by Methylation-specific PCR in HeLa and SiHa cell lines. M showed methylation-specific primer amplification, and U showed unmethylation-specific amplification. d Bisulfite genomic sequencing analysis was performed spanning the CCND2 AS1 core promoter and exon 1 in HeLa cell line. Twenty-two individual CpG sites within the CCND2 AS1 promoter were sequenced. Each row represents a single sequence; white circle indicates unmethylated CpG sites and black circle indicates methylated CpG sitesBack to article page