Fig. 6

HBP1 silence reverses the function of miR-203 on RASF growth and inflammation. RASFs were transfected with in-miR-NC, in-miR-203, in-miR-203 + si-NC, or in-miR-203 + si-HBP1. a, b Transfection efficiency was analyzed by using qRT-PCR or western blot. c, d The proliferation of RASFs was determined by colony formation assay and CCK-8 assay, respectively. e Flow cytometry was applied to detect the apoptosis of RASFs. f Western bolt analysis of Ki67, cleaved caspase3 and cleaved PARP expression in RASFs was carried out. g–j The level of inflammatory cytokines IL-6, IL-1β, IL-8 and TNF-α in RASFs was detected by ELISA assay. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 (ANOVA)