Fig. 5

Hsa_circ_0000231 reversed Sev-mediated influences on CRC development. A The impacts between Sev treatment and hsa_circ_0000231 overexpression on hsa_circ_0000231 expression were detected by qRT-PCR in HCT116 and SW620 cells. B, C The impacts between Sev treatment and hsa_circ_0000231 overexpression on the viability and colony-forming ability of HCT116 and SW620 cells were determined by MTT and cell colony formation assays, respectively. D DNA content quantitation assay was performed to reveal the effects between Sev treatment and hsa_circ_0000231 overexpression on cell cycle. E Annexin V-FITC and PI double staining assay was conducted to show the effects between Sev treatment and hsa_circ_0000231 overexpression on the apoptosis of HCT116 and SW620 cells. F Caspase-3 activity assay was used to demonstrate the impacts between Sev treatment and ectopic hsa_circ_0000231 expression on caspase-3 activity. G, H The impacts between Sev treatment and hsa_circ_0000231 overexpression on the migration and invasion of HCT116 and SW620 cells were revealed by wound-healing and transwell invasion assays, respectively. I The effects between Sev treatment and hsa_circ_0000231 overexpression on the protein expression of Bax, t-caspase 3, C-caspase 3, MMP2 and MMP9 were determined by western blot analysis in HCT116 and SW620 cells. *p < 0.05 (ANOVA with Tukey’s test)