Fig. 1From: PTD-mediated delivery of α-globin chain into Κ-562 erythroleukemia cells and α-thalassemic (HBH) patients’ RBCs ex vivo in the frame of Protein Replacement TherapySchematic illustration of the cloning procedure, starting with RT-PCR from placenta tissue, derived from a healthy individual, followed by TA cloning of the a-globin CDS and then TA cloning of amplified TAT-α-globin-HA and α-globin-HA into pCRII-TOPO vector. Then, «sticky ends» cloning was conducted, with the restriction enzymes NdeI and XhoI, to generate the recombinant expression vectors pET-16b-TAT-α-globin-HA and pET-16b-α-globin-HABack to article page