PCR products of the Datura variants amplified using the URP primers. Each lane is labeled according to the template and primer used for amplification. Letters represent variants followed by dot and a number that represents primer. Variant labels are S: D. stramonium; F: D. ferox; A: putative D. ferox × f. tatula; B: putative D. ferox × f. stramonium. URP primers used (1–12) are shown in Table 5. Molecular marker is the 1 kb DNA ladder.