In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons

Table 5 Characteristics of 12 URP and the P1 and P2 primers used for amplifying trnL intron

No	Primers	Sequences (5′-3′)	GC% content	Tm (°C)
1	URP1F	ATCCAAGGTCCGAGACAACC	55	65
2	URP2F	GTGTGCGATCAGTTGCTGGG	60	67
3	URP2R	CCCAGCAACTGATCGCACAC	60	65
4	URP4R	AGGACTCGATAACAGGCTCC	55	66
5	URP6R	GGCAAGCTGGTGGGAGGTAC	65	65
6	URP9F	ATGTGTGCGATCAGTTGCTG	50	67
7	URP13R	TACATCGCAAGTGACACAGG	50	68
8	URP17R	AATGTGGGCAAGCTGGTGGT	55	74
9	URP25F	GATGTGTTCTTGGAGCCTGT	50	65
10	URP30F	GGACAAGAAGAGGATGTGGA	50	65
11	URP32F	TACACGTCTCGATCTACAGG	50	65
12	URP38F	AAGAGGCATTCTACCACCAC	50	65
13	P1	CGAAATCGGTAGACGCTACG	55	63
14	P2	GGGGATAGAGGGACTTGAAC	55	62

ISSN: 2241-5793