Figure 2

Evidence of the expression of HIF-1α, VEGF, and CD133 proteins in mouse embryonic vasculogenesis. (A) Morphological observations of HIF-1α, VEGF and CD133 protein expression changed in E5.5-E9.5 with IHC staining (A1-A3, ×400; A4-A12, ×40). HIF-1α positive expression in embryo cells was identified in the cytoplasm as well as in nucleolus (A1). VEGF positive expression in embryo cells was identified in the cytoplasm (A2), CD133 positive expression in embryo cells was identified in the cytoplasm and membrane (A3). A marked change between the inner and outer zones can be observed in the figure (A4-A12). (B) Morphological observations of CD133 and VEGF protein expression showed an irregular liner pattern in E6.5-E7.5 and the pattern was similar to endothelium-dependent vessels distribution with IHC staining (B1-B2, ×200). (C) The bar diagram represents the changed in the expression of the three proteins with time from E5.5 to E9.5 in the inner (C1) and outer zones (C2), respectively (p < 0.05).