Figure 3
From: Expression of relative-protein of hypoxia-inducible factor-1α in vasculogenesis of mouse embryo
Evidence of the expression of Twist, E-cadherin, and Vimentin proteins in mouse embryonic vasculogenesis. (A) Morphological observation of the changes in the expression of Twist, E-cadherin, and Vimentin protein expression from E5.5 to E9.5 with IHC staining (A1-A3, ×400; A4-A12, ×40). Twist positive expression in embryo cells was identified in the cytoplasm as well as in nucleolus (A1). E-cadherin positive expression in embryo cells was identified in the cytoplasm and membrane (A2). Vimentin positive expression in embryo cells was identified in the interstitial tissue (A3). The marked change between the inner and outer zones can be observed in the figure (A6-A12). (B) Morphological observation of Twist protein expression in the adjacent area of vessels (B1, ×400). Morphological observation of Twist, E-cadherin, and Vimentin protein expression showed a patchy pattern during E6.5-E7.5 with IHC staining (B2-B4, ×200). (C) The bar diagram represents the changed in the expression of the three proteins with time from E5.5 to E9.5 in the inner (C1) and outer zones (C2), respectively (p < 0.05).