Figure 2

Analysis of GFP expression in primary satellite cells transfected by pEGFP-N1 plasmid. GFP fluorescence in PSCs transfected by pEGFP-N1 plasmid as visualized by fluorescent microscopy. (A) Transfected ovine fetus PSCs 24 hrs, (B) 48 hrs after first transfection (C) and 48 hrs after second transfection. (D) FACS analysis of GFP expression level in a time lapse. The y-axis shows mean of the expression levels and x-axis shows days after transfection. EGFP expression levels for transfected cells and cells number were quantified by measuring the extracellular EGFP by a fluorescence detector for 24 hrs and 48 hrs of first transfection, 3 days post-first transfection and 48 hrs after second transfection on days 1, 2, 5 and 7, respectively. FACS Analysis of GFP expression level for cell number showed that the percentage of transfected cells increased as increasing the transfection time.