Figure 6
From: Cell culture, sex determination and single cell cloning of ovine transgenic satellite cells in vitro
The sex determination results of ovine primary satellite cells using the duplex PCR system. Lane m and f are male and female blood samples, respectively; lane M is 50 bp DNA ladder; lanes 1–9 are male cells samples; lanes 10–15 are female cells samples; upper band (298 bp) corresponds to the positive control beta-actin product and it is present in all cells. Lower band (162 bp) corresponds to the HMG-box product and it is present only in males.