Figure 9
From: Cell culture, sex determination and single cell cloning of ovine transgenic satellite cells in vitro
Restriction patterns from Sac I digested ZFY/ZFX-PCR products of primary satellite cells samples. Lane m and f are male and female blood samples, respectively; lane M is 50 bp DNA ladder; lanes 1–9 are PCR products of male cells samples; lanes 10–15 are PCR product of female cells samples. The ZFY fragment remained uncut while the ZFX homologue (which is present in both male and female cells) was digested to give fragments of 172 and 273 bp.