Fig. 3

Dot plot and histogram of X- and Y-chromosomes after Hoechst 33342 staining for sex sorting in frozen-thawed bovine sperm. Frozen-thawed sperm was automatically sorted into X- and Y-sperm populations by flow cytometry. The sex-sorted sperm based on differences of DNA contents. A dot plot is displaying Hoechst 33342 fluorescent intensity versus side-angle scatter (SSC, a) and forward angle scatter (FSC, b). Histogram is displaying Hoechst 33342 fluorescent intensity of sorted X- and Y-sperm (c)