Fig. 4From: Effect of methyl-beta-cyclodextrin on the viability and acrosome damage of sex-sorted sperm in frozen-thawed bovine semenHoechest 33342-dyed sperms were separated to X and Y populations based on differences of DNA content by flow cytometry. Green spots fluorescent intensity in X chromosome sperm. Blue spots fluorescent intensity in Y chromosome sperm (top). The frozen-thawed X and Y sperm dyed with SYBR-14 and PI, and viability was analyzed by fluorescence intensity in flow cytometric dot plot (bottom). a–i Different letters are significantly different in viability of X (a–e) and Y (f–i) sperm (p < 0.05). *Different letters are significantly difference in viability between X and Y sperm (p < 0.05)Back to article page