Fig. 2

Calibration curves for CYP2E1 specific tryptic peptides YPEIEEK (P1) and GTVVVPTLDSVLYDNQEFPDPEK (P2). a YPEIEEK calibration curve was constructed from serial dilutions of synthetic peptides and spiked in pooled plasma from control subjects containing 50 fg µL⁻¹ of deuterated YPEIEEK as internal standard. Each data point is mean of triplicates and error bars represent standard deviation (SD) from the mean. The calibration curve showed linearity (R² = 0.997) between concentrations 0–1000 fg µL⁻¹. b GTVVVPTLDSVLYDNQEFPDPEK calibration curve was constructed from serial dilutions of synthetic peptides and spiked in pooled plasma from control subjects containing 50 fg µL⁻¹ of deuterated YPEIEEK as internal standard. Each data point is mean of triplicates and error bars represents standard deviation (SD) from the mean. The calibration curve showed linearity (R² = 0.989) between concentrations 0–1000 fg µL⁻¹