Table 1 Primer sequences, amplicon characteristics and expected amplicon size in DNA contamination assay
Amplicon (primer pair) name | Amplifying region | Sequence | Expected amplicon size (bp) |
|---|---|---|---|
SSU-5.8S | Amplify partial 17–18S rRNA, complete ITS1 and partial 5.8 S rRNA | SF†: cgtaacaaggtttccgtaggtg R*: ggttcacgggattctgcaat | 332–405 |
5.8S-LSU | Amplify partial 5.8 S rRNA complete ITS2 and partial 25–28S rRNA, | F*: attgcagaatcccgtgaacc LR†: tgcttaaactcagcgggtag | 318–361 |
ITS1 | Contains internal transcribed spacer 1 | ggtatggcgtcaaggaacact atagcatcgctgcaagaggt | 100–200 |