DNA methylation changes and TE activity induced in tissue cultures of barley (Hordeum vulgare L.)

Table 4 The metAFLP, SSAP and MSTD primer sequences

Selective primer	Selective primer combinations 5′→3′
	MseI directed primer sequences
MCAA	GAT GAG TCC TGA GTA ACA A
MCAG	GAT GAG TCC TGA GTA ACA G
MCCA	GAT GAG TCC TGA GTA ACC A
MCCG	GAT GAG TCC TGA GTA ACC G
MCGT	GAT GAG TCC TGA GTA ACG T
MCTC	GAT GAG TCC TGA GTA ACT C
	metAFLP directed selective primer sequences
CpXpG-AGC	CAT GCG TAC AGT ACC AGC
CpG-GCA	CA TGC GTA CAG TAC CGC A
	TE directed selective primer sequences
BARE1 E0377	TGT TGG AAT TAT GCC CTA GAG G
BARE LO45C	TGT TTC CCA TGC GAC GTT CC
BARE LTR	CTA GGG CAT AAT TCC AAC A
BARE1 E1814	TTG CCA TGC GAC GTT CCC CAA C
BARE1 92460	CTG GCT AGC CAA CTA GAG GCT TGC
BARE1 81078	ATC ATT GCC TCT AGG GCA TAA TTC C
SUKKULA 9900	GAT AGG GTC GCA TCT TGG GCG TGA C
SUKKULA E0229	ACG TCG GCA TCG GGC TGT CAC
SUKKULA E0228	GGA ACG TCG GCA TCG GGC TG
NIKITA E2611	TGG GAT CAC TTG ATC CCT CTC G
NIKITA	AAG AAG TGC CTA TGG ACA AAT CC

ISSN: 2241-5793