Fig. 4

TaqMan analysis of bone resorption molecules: RANK, RANKL and OPG expression in calvariae of C57BL/KalwRiJHsD mice. RNA was extracted with cDNA synthesized from frontal (F), parietal (P) and interparietal (IP) bones. The relative expression of osteoclast marker genes was adjusted to the relative expression of a housekeeping gene, β-actin. Data show that the RANK gene was only expressed in interparietal bone, while it was not expressed in frontal or parietal bones. Additionally, there was a significant increase in the relative expression of the RANK gene (a) in interparietal bone compared to frontal and parietal bones. On the other hand, there was a significant increase in the relative expression of the RANKL (b) and OPG (c) genes in parietal bone compared to frontal and interparietal bones