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Table 1 Endpoint PCR data

From: Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection

Storage time (h) Gene expression in CD45-negative cells from healthy individuals
18S rRNA ACTB EPCAM KRT19 PECAM1 CDH2
0 + + +
24 + + +
48 + + +
72 + + +
96 + + +
Storage time (h) Gene expression in breast-cancer cells spiked into blood from healthy individuals
18S rRNA ACTB EPCAM KRT19 PECAM1 CDH2
0 + + + + +
24 + + + + +
48 + + + + +
72 + + + + +
96 + + +
Storage time (h) Gene expression in colon-cancer cells spiked into blood from healthy individuals
18S rRNA ACTB EPCAM KRT19 PECAM1 CDH2
0 + + + + +
24 + + + + +
48 + + + + +
72 + + + + +
96 + + +
  1. Expression of genes was determined electrophoretically by the presence of a product band at the end of PCR. “+” indicates expression, whereas “−” indicates the absence of expression