Table 4 Primers used in PCR experiments
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
18S rRNA | TGCCCTATCAACTTTCGATGGTAGTC | TTGGATGTGGTAGCCGTTTCTCA |
28S rRNA | CGGGTAAACGGCGGGAGTAAC | TGGCTGTGGTTTCGCTGGATAG |
ACTB | GCCCTGGACTTCGAGCAAGAGA | CAGGAAGGAAGGCTGGAAGAGTG |
NANOG | CGTGTGAAGATGAGTGAAACTG | GGATGGGCATCATGGAAA |
POU5F1 | AGGAAGCTGACAACAATG | ACTCGGTTCTCGATACTG |
CD34 | CCCATGCTGGAGGTGACATCTC | CCAGGGAGCCGAATGTGTAAAG |
NES | GAGACACCTGTGCCAGCCTTTCTTA | CTGGGCTCTGATCTCTGCATCTACAG |
SOX2 | CTCGCCCACCTACAGCAT | GCTGGCCTCGGACTTGAC |
cFOS | CCTTCACCCTGCCTCTCCTCAAT | GCCTGGATGATGCTGGGAACA |
cJUN | CCAACTCATGCTAACGCAGCAGTT | ACCCTTGGCTTTAGTTCTCGGACAC |
cMET | AACAGGTGCAAAGCTGCCAGTG | GCACGCCAAAGGACCACACAT |
DHFR | AGTCAGCGAGCAGGTTCTCATTGA | TGGACTATGTTCCGCCCACACA |
EGFR | CCCCTGACTCCGTCCAGTATTGA | CTTTTCCTCCAGAGCCCGACTC |
ERCC1 | GCTACCACAACCTGCACCCAGACT | GCAGTCGGCCAGGATACACATCT |
EPCAM | ACTGCTGGGGTCAGAAGAACAGAC | TCTGAAGTGCAGTCCGCAAACTT |
PECAM1 | GGTCAGCAGCATCGTGGTCAAC | GGATGGAGCAGGACAGGTTCAGT |
CDH2 | CGTGAAGGTTTGCCAGTGTGACTC | TTATCCCGGCGTTTCATCCATAC |
KRT19 | AGCGGCTCATGGACATCAAGTC | AGGACAGCAGAAGCCCCAGAG |