Genetic diversity analysis of cultivated Korarima [Aframomum corrorima (Braun) P.C.M. Jansen] populations from southwestern Ethiopia using inter simple sequence repeats (ISSR) marker

Table 2 List of primers, their sequence, annealing temperature and amplification pattern used for PCR optimization

	Primer	Primer sequences	Annealing temperature (°C)	Amplification pattern
1	UBC-810	(CA)₈T	45	No band
2	UBC-811	(CA) ₈ RC	48	Very good
3	UBC-813	(GATA)₈	45	Fair
4	UBC-814	(AT)₈YC	45	No band
5	UBC-815	(GA)₈YT	48	Fair
6	UBC-817	(GT)₈YA	45	Fair
7	UBC-822	(GT)₈YC	45	No band
8	UBC-825	(AT) ₈ T	45	Very good
9	UBC-831	(AT)₈YA	48	No band
10	UBC-834	(AG) ₈ YT	45	Excellent
11	UBC-835	(AG) ₈ YC	48	Excellent
12	UBC-841	(GA) ₈ YC	48	Excellent
13	UBC-843	(CT)₈RA	45	Fair
14	UBC-846	(CA)₈RT	48	No band
15	UBC-849	(GT)₈YA	45	No band
16	UBC-850	(GT)₈YC	48	Fair
17	UBC-853	(TC) ₈ RT	48	Good
18	UBC-857	(AC) ₈ YG	45	Very good

ISSR primers (UBC 1–18) were from the University of British Columbia, Canada. The seven primers used in the present study for PCR amplification are highlighted in italics