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Fig. 5 | Journal of Biological Research-Thessaloniki

Fig. 5

From: Molecular, biochemical and kinetic analysis of a novel, thermostable lipase (LipSm) from Stenotrophomonas maltophilia Psi-1, the first member of a new bacterial lipase family (XVIII)

Fig. 5

SDS-PAGE. a The whole cell protein extracts of two clones during overexpression, where in lane 1: MM marker; lanes 2, 5: BL21(DE3)/pET29c; lanes 3, 6: BL21(DE3)/pET29c::lip clone a; lanes 4, 7: BL21(DE3)/pET29c::lip clone b, b lipase purification steps, where in lane 1: MM marker; lane 2: crude protein extract at 24 h induction; lane 3: total soluble protein extract after cell lysis and centrifugation; lane 4: purified lipase LipSm fraction 1; lane 5: purified lipase LipSm fraction 2; lane 6: purified lipase LipSm fraction 3

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Journal of Biological Research-Thessaloniki

ISSN: 2241-5793

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