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Fig. 4 | Journal of Biological Research-Thessaloniki

Fig. 4

From: Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin

Fig. 4

Cortical microtubule orientation in cortex cells of wild-type (a, e) and p60-katanin mutants (bd, fh). All high magnification images (eh) are maximum projections of serial CLSM sections through the cortical cytoplasm. Cortex cell files, derived from the areas defined by rectangular frames on low magnification images (ad), were released after application of gentle pressure on the whole mount root specimens. Fairly transverse cortical microtubules can be observed in Col-0 cells (e). In ktn1-2 (f), lue1 (g) and fra2 (h), though a general transverse pattern exists, several microtubules exhibit random orientation, while foci of microtubule convergence can be discerned as well. Representative decipher-graphs of microtubule alignment in meristematic cortex cells of Col-0 (i), ktn1-2 (j), lue1 (k) and fra2 (l). Generally, transverse microtubule orientation prevailed in all the cases (doubled-headed arrow). However, in the katanin mutants an increase in the frequency of longitudinal and/or randomly oriented microtubules was noticed. Scale bars 50 μm (ad), 10 μm (eh)

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