Fig. 4

Electron micrographs of Golgi stacks in high pressure frozen/freeze-substituted Venus flytrap gland cells. Note the increase in size of the inflated cisternal margins of the Golgi-associated trans Golgi network (GA-TGN) and the free TGN elements and of the size of the secretory vesicles (SV) in the 4 and 6 day samples compared to the control and 1 day samples. Peeling GA-TGN cisternae are seen in a–c, and free TGN cisternae in a, c and d. In b, a COPII bud is seen at an ER export site adjacent to the cis-side of the Golgi stack, and COPIa- and COPIb-type vesicles are associated with cis and medial Golgi cisternae. Bars 0.2 µm