Fig. 5

Tomographic slice image and corresponding tomographic model of a Golgi stack and TGN cisternae with associated actin-like filaments (arrows) in a 1 day-induced gland cell. a Tomographic slice image illustrating differences in architecture and staining of cis, medial and trans Golgi, Golgi-associated trans Golgi network (GA-TGN) and free trans Golgi network TGN cisternae, an ER cisterna with a budding COPII vesicle (ER/COPII), clathrin-coated vesicles (CCV), and secretory vesicles (SV). The actin-like filaments associated with the free TGN cisternae are a unique feature of the 1 day BSA-induced cells. b 3D tomographic model of the structures illustrated in a. The filaments are organized in the form of a loose bundle around the free TGN cisternae (only one 1 day-induced sample was analyzed in this study). Vacuole (V). Bars 0.1 µm