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Table 2 Component loadings and mean principal component (PC) scores, showing the association between the total and constituent anthocyanin contents and the expression levels of the biosynthetic and regulatory genes, as determined using a principal component analysis

From: Transcriptional regulation of anthocyanin biosynthesis in a high-anthocyanin resynthesized Brassica napus cultivar

Variable PC1 PC2 PC3
Upstream genes
 PAL1 0.14 0.29 0.24
 PAL2 − 0.06 0.36 0.26
 C4H − 0.09 0.34 0.24
 4CL1 − 0.04 0.37 0.25
 CHS 0.22 − 0.01 − 0.19
 CHI 0.23 0.01 − 0.09
 F3H 0.21 − 0.05 0.19
 F3´H 0.23 − 0.08 − 0.03
Downstream genes
 FLS1 0.23 0.02 0.11
 DFR 0.23 − 0.03 0.15
 ANS 0.23 − 0.08 0.12
 UGT75C1 0.22 − 0.01 − 0.17
 UGT78D2 0.23 0.03 0.10
Regulatory genes
 MYB12 0.23 0.04 − 0.05
 MYB111 0.22 − 0.03 − 0.22
 PAP2 0.22 0.08 0.10
 MYB113 0.23 0.01 − 0.05
 BrTT8 − 0.11 − 0.34 0.17
 TTG1 0.20 0.11 − 0.15
 TT19 0.22 − 0.03 0.21
Anthocyanin components
 C3-dig5-gl − 0.04 − 0.38 0.23
 C3-s-dig5-gl 0.21 − 0.10 0.28
 C3-sop5-m.gl 0.23 − 0.01 − 0.07
 Mal3-(p-c)gl py de 0.21 − 0.03 0.28
 Pe3-(6-ac)-gl 0.20 0.01 − 0.37
 C3-(p-c)digl5-gl − 0.12 − 0.33 0.15
Total anthocyanin 0.09 − 0.35 0.23
Eigenvalue 18.22 6.07 2.10
% of the variation explained 67.5 22.5 7.0
Cumulative % of the variation explained 67.5 90.0 97.0
Genotype Mean PC score ± SD
 Asia 0.57 ± 0.09 B 0.36 ± 0.12 B − 2.37 ± 0.20 C
 Rs306 − 3.31 ± 0.02 C 3.28 ± 0.11 A 0.89 ± 0.09 A
 Rs035 6.45 ± 0.04 A − 0.29 ± 0.08 C 0.96 ± 0.08 A
 Rubea − 3.72 ± 0.02 D − 3.35 ± 0.07 D 0.52 ± 0.04 B
  1. SD standard deviation, C3-dig5-gl cyanidin 3-diglucoside-5-glucoside, C3-s-dig5-gl cyanidin 3-(sinapoyl)-diglucoside-5-glucoside, C3-sop5-m.gl cyanidin 3-sophoroside-5-malonylglucoside, Mal3-(p-c)gl py de malvidin-3-(p-coumaroyl)glucoside pyruvic derivative, Pe3-(6-ac)-gl pelargonidin-3-(6-acetyl)-glucoside, C3-(p-c)digl5-gl cyanidin 3-(p-coumaroyl) diglucoside-5-glucoside
  2. Letters in mean PC score against the genotypes are mean separation using Tukey test with p < 0.05