Fig. 2
From: Immune cell counts and signaling in body fluids of cows vaccinated against Clostridium difficile
Total PBL contents and the ratios of phagocytes to lymphocytes in blood. On the ordinates, the total PBL contents and the ratios of phagocytes to lymphocytes in blood are depicted as LSM ± SD for the three examined animal groups [(“Ctr” control animals (n = 5), “LR” treated, low responder animals (n = 4), “HR” treated, high responder animals (n = 5)]. The physiological range of PBL (5 up to 10 million leukocytes per milliliter blood) was accented by two gray dotted lines. The 31-week treatment period (TW) is depicted on the lower abscissa. The upper abscissa indicates the different application routes [(nasal (N), percutaneous (PC), subcutaneous (SC) and intracutaneous (IC)] of the vaccines to the TWs with immunization (IM). Only once, incomplete Freund’s adjuvant (iFA) was supplemented to the MucoCD-I vaccine. The gray bars in TWs 3 and 17 highlight the times of the first uses of the vaccine batches A and B, respectively. During the treatment periods with both vaccine batches and the particular TW before the first use of vaccine batch A or B, substantial differences within the same group are marked by asterisks (p < 0.05). Lower case (a, b, c) label evident differences (p < 0.05) between the groups at the same dates