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Table 1 Classification of the gene regulation expressed as ΔΔCq range

From: Immune cell counts and signaling in body fluids of cows vaccinated against Clostridium difficile

Defined grades of gene regulation

ΔΔCq range

0

Non-regulated

| < 1|

+

Weak

| ≥ 1| up to | < 3|

++

Strong

| ≥ 3| up to | < 5|

+++

Very strong

| ≥ 5|

  1. The grades of gene regulation are given as follows: “0” for non-regulated, “+” for weakly regulated, “++” for strongly regulated, “+++” for very strongly regulated