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Table 2 Spectrum of genes analyzed in PBL and in SCC out of milk and the classification of their regulation

From: Immune cell counts and signaling in body fluids of cows vaccinated against Clostridium difficile

Genes Performed gene expression analysis Classification of the gene regulation
In PBL In SCC In PBL In SCC
bMEC′-related genes
 KRT8 No Yes / +
 PIGRa No Yes / ++
 FcRNa No Yes / 0
 C1QAa No Yes / 0
 C3a No Yes / +
 CCL5a No Yes / +
 CCL20a No yes / ++
 CCL28a No Yes / +
 CXCL3a No Yes / +
 CXCL5a No Yes / 0
 CXCL8a No Yes / +
 LPOa No Yes / ++
 LFa No Yes / +
 LYZ1a No Yes / +
 TAPa No Yes / +
Phagocyte-related genes
 C3AR1 Yes Yes + +
 C5AR1 Yes Yes + ++
 CXCR2 Yes Yes + +
 TLR2 Yes Yes + +
 CD163 Yes Yes + 0
 CXCL8 Yes See above + See above
 TNFα Yes Yes + ++
 IL1β Yes Yes ++ +
 IL6 Yes Yes +++ +
 IL12β Yes Yes ++ +
Lymphocyte-related genes
 CD3δ Yes Yes +++ +
 CD4 Yes Yes ++ ++
 CD8β Yes Yes ++ +
 CD19 Yes Yes + +
 CD38 No Yes / +
 CD126 Yes Yes +++ +
 CCR6 No Yes / +
 IFNγ Yes Yes + +
 TGFβ1 Yes No + /
 IL2 Yes No +++ /
 IL10 Yes Yes + ++
  1. PBL peripheral blood leukocytes, SCC somatic cell count, bMEC bovine epithelial cells
  2. a characterizes the bMEC-related genes, which were additionally normalized with KRT8 as the epithelial cell marker; the sign / means that the gene expression was not analyzed in the study objects, PBL or SCC