Immune cell counts and signaling in body fluids of cows vaccinated against Clostridium difficile

Table 2 Spectrum of genes analyzed in PBL and in SCC out of milk and the classification of their regulation

Genes	Performed gene expression analysis		Classification of the gene regulation
Genes	In PBL	In SCC	In PBL	In SCC
bMEC′-related genes
KRT8	No	Yes	/	+
PIGR^a	No	Yes	/	++
FcRN^a	No	Yes	/	0
C1QA^a	No	Yes	/	0
C3^a	No	Yes	/	+
CCL5^a	No	Yes	/	+
CCL20^a	No	yes	/	++
CCL28^a	No	Yes	/	+
CXCL3^a	No	Yes	/	+
CXCL5^a	No	Yes	/	0
CXCL8^a	No	Yes	/	+
LPO^a	No	Yes	/	++
LF^a	No	Yes	/	+
LYZ1^a	No	Yes	/	+
TAP^a	No	Yes	/	+
Phagocyte-related genes
C3AR1	Yes	Yes	+	+
C5AR1	Yes	Yes	+	++
CXCR2	Yes	Yes	+	+
TLR2	Yes	Yes	+	+
CD163	Yes	Yes	+	0
CXCL8	Yes	See above	+	See above
TNFα	Yes	Yes	+	++
IL1β	Yes	Yes	++	+
IL6	Yes	Yes	+++	+
IL12β	Yes	Yes	++	+
Lymphocyte-related genes
CD3δ	Yes	Yes	+++	+
CD4	Yes	Yes	++	++
CD8β	Yes	Yes	++	+
CD19	Yes	Yes	+	+
CD38	No	Yes	/	+
CD126	Yes	Yes	+++	+
CCR6	No	Yes	/	+
IFNγ	Yes	Yes	+	+
TGFβ1	Yes	No	+	/
IL2	Yes	No	+++	/
IL10	Yes	Yes	+	++

PBL peripheral blood leukocytes, SCC somatic cell count, bMEC bovine epithelial cells
^a characterizes the bMEC-related genes, which were additionally normalized with KRT8 as the epithelial cell marker; the sign / means that the gene expression was not analyzed in the study objects, PBL or SCC

ISSN: 2241-5793