Fig. 1

Map of LV DNA in the LV shuttle plasmids. pLV.Tbx20 (a), pLV.Ctrl (b). 5′ LTR chimeric 5′ long terminal repeat including the Rous sarcoma virus U3 region as well as the HIV1 R and U5 regions. Ψ HIV1 packaging signal, RRE HIV1 Rev-responsive element, cPPT HIV1 central polypurine tract and termination site, hCMV-IE human cytomegalovirus immediate early gene promoter, Tbx20 coding sequence of desired gene, EMCV IRES encephalomyocarditis virus internal ribosomal entry site, EGFP enhanced green fluorescent coding sequence, hHBVPRE human hepatitis B virus posttranscriptional regulatory element, 3′ LTR 3′ HIV1 long terminal repeat involving a deletion in the U3 region to render the LV self-inactivating