Table 1 RT-qPCR primers

ALPL

Alkaline phosphatase

F: 5′-CATGCTGAGTGACACAGACAAGAAG-3′

R: 5′-TGGTAGTTGTTGTGAGCATAGTCCA-3′

COL1A1

Collagen type I alpha 1

F: 5′-CATCTCCCCTTCGTTTTTGA-3′

R: 5′-CCAAATCCGATGTTTCTGCT-3′

RUNX2

Runt-related transcription factor 2

F: 5′-GGAGTGGACGAGGCAAGAGTTT-3′

R: 5′-AGCTTCTGTCTGTGCCTTCTGG-3′

OPN

Osteopontin

F: 5′-ATGATGGCCGAGGTGATAGT-3′

R: 5′-ACCATTCAACTCCTCGCTTT-3′

Tbx20 (human)

Homo sapiens T-box 20

F: 5′-CAAGCCCCAACTCTCCTCTC-3′

R: 5′-CTCCACCAAACTCCCCATGA-3′

Tbx20 (murine)

Murine T-box20

F: 5′-ATACCGCTATGCCTACCACC-3′

R: 5′-TATGGCCGTGTTGATCCAGT-3′

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

F: 5′-ATGTTCGTCATGGGTGTGAAC-3′

R: 5′-CACAGTCTTCTGGGTGGCAG-3′