Table 2 List of ISSR primers along with their annealing temperature, respective sequences and amplification efficiency used during optimization

1

UBC-812

47 °C

(GA)8A

Excellent

2

UBC-817

48 °C

(CA)8A

Poor

3

UBC-818

47 °C

(CA)8G

No band

4

UBC-826

47 °C

(AC)8C

No band

5

UBC-834

47 °C

(CA)8AG

Excellent

6

UBC-835

48 °C

(AG)8YC

Very good

7

UBC-839

49 °C

(TA)8RG

Poor

8

UBC-841

48 °C

(GA)8YC

Excellent

9

UBC-844

48 °C

(AG)8YT

Excellent

10

UBC-848

48 °C

(CA)8RG

No band

11

UBC-851

49 °C

(GT)8YG

Poor

12

UBC-852

48 °C

(AC)8T

Poor

13

UBC-854

49 °C

(TC)8RG

Excellent

14

UBC-857

49 °C

(AC)8AYG

Excellent

15

UBC-860

52 °C

(TG)8RA

Poor

16

UBC-864

48 °C

(CA)8RT

Very poor

17

UBC-865

47 °C

(CCG)6

Poor

18

UBC-873

45 °C

(GACA)4

No band

19

UBC-878

48 °C

(GGAT)4

Poor

20

UBC-879

48 °C

(CTTCA)3

Poor

21

UBC-866

55 °C

(CTC)6

Good

22

UBC-868

55 °C

(GAA)6

Excellent

23

UBC-880

48 °C

(GGAGA)3

Excellent

24

UBC-881

49 °C

(GGTG)3

No band

25

UBC-888

47 °C

BDB(CA)7

Poor