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Table 2 List of ISSR primers along with their annealing temperature, respective sequences and amplification efficiency used during optimization

From: Genetic diversity analysis in Plectranthus edulis (Vatke) Agnew populations collected from diverse geographic regions in Ethiopia using inter-simple sequence repeats (ISSRs) DNA marker system

S/N Primer Annealing temperature (°C) Primer sequences Amplification efficiency
1 UBC-812 47 °C (GA)8A Excellent
2 UBC-817 48 °C (CA)8A Poor
3 UBC-818 47 °C (CA)8G No band
4 UBC-826 47 °C (AC)8C No band
5 UBC-834 47 °C (CA)8AG Excellent
6 UBC-835 48 °C (AG)8YC Very good
7 UBC-839 49 °C (TA)8RG Poor
8 UBC-841 48 °C (GA)8YC Excellent
9 UBC-844 48 °C (AG)8YT Excellent
10 UBC-848 48 °C (CA)8RG No band
11 UBC-851 49 °C (GT)8YG Poor
12 UBC-852 48 °C (AC)8T Poor
13 UBC-854 49 °C (TC)8RG Excellent
14 UBC-857 49 °C (AC)8AYG Excellent
15 UBC-860 52 °C (TG)8RA Poor
16 UBC-864 48 °C (CA)8RT Very poor
17 UBC-865 47 °C (CCG)6 Poor
18 UBC-873 45 °C (GACA)4 No band
19 UBC-878 48 °C (GGAT)4 Poor
20 UBC-879 48 °C (CTTCA)3 Poor
21 UBC-866 55 °C (CTC)6 Good
22 UBC-868 55 °C (GAA)6 Excellent
23 UBC-880 48 °C (GGAGA)3 Excellent
24 UBC-881 49 °C (GGTG)3 No band
25 UBC-888 47 °C BDB(CA)7 Poor
  1. Source: Primer kit 900 (UBC 900); Single-letter abbreviations for mixed base positions: R = (A, G); Y = (C, T)