Fig. 2

Aβ treatment increased the levels of inflammatory factors in SH-SY5Y cells. SH-SY5Y cells were treated with different concentrations (0, 10, 25, 50 μM) of Aβ for 48 h. a–c ELISA assay was carried out to measure the concentration of IL-1β, TNF-αand IL-6 in the culture supernatant. d–f qRT-PCR analysis was conducted to detect the mRNA expression of IL-1β, TNF-α and IL-6 in SH-SY5Y cells. *p < 0.05 vs. controls