Fig. 1
From: Modulation of RECK levels in Xenopus A6 cells: effects on MT1-MMP, MMP-2 and pERK levels
Treatment of A6 cells with RECK MO resulted in decreased RECK protein levels. Immunoblot analysis was used to confirm reduction of RECK protein following treatment of A6 cells with RECK MO using Endo-porter delivery reagent. Protein was extracted from cells 48 h following treatment with increasing concentrations of RECK MO. a Knockdown of RECK protein occurred in a dose-dependent manner. β-actin is used as a loading control. b Quantification of protein levels in a were graphed and data is based on 3 biological replicates (mean ± SD) and normalized to control (set to 100%). Data is analyzed via t test; *, p ≤ 0.05; ***, p ≤ 0.001; ****, p ≤ 0.0001