Fig. 2

RECK reduction did not alter MT1-MMP or pERK protein levels nor MMP-2 activity levels. a Densitometry quantification of MT1-MMP immunoblotted protein normalized to β-actin. MT1-MMP protein levels did not significantly change in RECK knockdown cells compared to control (set to 1). β-actin is used as a loading control. b Densitometry quantification of pERK normalized to total ERK levels. pERK protein levels did not significantly change in RECK reduced cells compared to control (set to 1). c Gelatin zymography was used to measure protein activity of secreted pro-, intermediate, and active MMP-2 following RECK knockdown in A6 cells. Data is presented as the ratio between active and total (pro-, intermediate, and active) MMP-2 levels between control and RECK reduced cells. Active/total MMP-2 levels did not significantly change in the media of RECK reduced cells compared to control (set to 1). Secreted MMP-9 protein could not be detected at the expected size of 92 kDa (shown by the arrow). Graphed data is based on 3 biological replicates (mean ± SD). Data is analyzed via t-test; ns, p > 0.05