Fig. 4

RECK overexpression increased MT1-MMP protein and MMP-2 activity levels and decreased pERK protein levels. a Densitometry quantification of immunoblots of MT1-MMP protein normalized to β-actin. MT1-MMP protein levels significantly increased in RECK-overexpressing cells compared to control (set to 1). b Densitometry quantification of pERK normalized to total ERK levels. pERK protein levels significantly decreased in RECK-overexpressing cells compared to control (set to 1). c Gelatin zymography was used to measure protein levels of secreted pro-, intermediate, and active MMP-2 following RECK overexpression in A6 cells. Data is presented as the ratio between active and total (pro-, intermediate, and active) MMP-2 levels between control and RECK-overexpressing cells. Active/total MMP-2 levels significantly increased in the media of RECK-overexpressing cells compared to control (set to 1). Secreted MMP-9 protein could not be detected at the expected size of 92 kDa (shown by the arrow). Graphed data is based on 3 biological replicates (mean ± SD). Data is analyzed via t-test; *, p ≤ 0.05; **, p ≤ 0.01