Fig. 6

PI-PLC treatment caused an increase in MT1-MMP protein and MMP-2 activity levels. a Densitometry quantification of MT1-MMP protein level normalized to β-actin in control A6 cells treated with PI-PLC. MT1-MMP protein levels significantly increased in PI-PLC-treated cells compared to untreated cells (set to 1). b Densitometry quantification of pERK normalized to total ERK levels. pERK protein levels did not significantly change following PI-PLC treatment. c Gelatin zymography was used to measure protein levels of secreted pro-, intermediate, and active MMP-2 following PI-PLC treatment in A6 cells. Data is presented as the ratio between active and total (pro-, intermediate, and active) MMP-2 levels between untreated cells and PI-PLC treated cells. Active/total MMP-2 levels significantly increased in the media of PI-PLC-treated cells compared to untreated cells (set to 1). Secreted MMP-9 protein could not be detected at the expected size of 92 kDa (shown by the arrow). Graphed data is based on 3 biological replicates (mean ± SD). Data is analyzed via t-test; ns, p > 0.05; *, p ≤ 0.05; **, p ≤ 0.01