Fig. 3
From: TRAF3 can interact with GMEB1 and modulate its anti-apoptotic function
The effect of TRAF3 on GMEB1-mediated antiapoptotic function. a Assessment of viability of HeLa cells treated by CHX and TNFα, using MTT assay. TRAF3 or TRAF3 M7 (0.5 μg) and GMEB1 (1 μg) expressing vectors were cotransfected to HeLa cells. 20 h postransfection, HeLa cells were treated with CHX and TNFα for 24 h, in order to induce apoptosis. Viability is calculated as the ratio of O.D. sample/O.D. control untreated * 100. The data are presented as the mean ± standard error (± SEM) of three independent repetitions. Statistical evaluation of the differences between the values was performed by Student’s t-test. Statistically significant differences are indicated by brackets and an asterisk (p < 0.05). b Immunoblot analysis. Total cell lysates from HeLa cells transfected with the vectors expressing the indicated proteins were analyzed by SDS-PAGE on a 8% gel. The detection of a catalytically inactive mutant (TRAF3 M7-flag) and the GMEB1-His was performed using the antibodies that are reported in “Methods” section. β-actin was used as a loading control