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Fig. 5 | Journal of Biological Research-Thessaloniki

Fig. 5

From: TRAF3 can interact with GMEB1 and modulate its anti-apoptotic function

Fig. 5

The effect of LMP(1–231) on GMEB1-mediated antiapoptotic function. a Assessment of viability of HeLa cells, treated by CHX and TNFα, using MTT assay. LMP(1–231) (0.5 μg) expression vector and GMEB1-His (1 μg) were co-transfected to HeLa cells. 20 h postransfection, HeLa cells were treated with CHX and TNF-α for 24 h, in order to induce apoptosis. Viability is calculated as the ratio of O.D. sample/O.D. control untreated * 100. The data are presented as the mean ± standard error (± SEM) of three independent repetitions. Statistical evaluation of the differences between the values was performed by Student’s t-test. Statistically significant differences are indicated by brackets and an asterisk (p < 0.05). b Immunoblot analysis. Total cell lysates from HeLa cells transfected with LMP(1–231) and GMEB1-His were analyzed by SDS-PAGE on an 8% gel. The detection of the indicated proteins was performed using the antibodies reported in “Methods” section. β-actin was used as a loading control

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