Fig. 6

Immunoprecipitation of total mouse brain proteins with Fab 65. 12% polyacrylamide gel was stained with silver ions. Lane 1: 15 μl of the immunoprecipitation reaction in the presence of mouse brain cell lysate and recombinant Fab 65 immobilized on protein L agarose beads. Lane 2: 15 μl of the pre-clearing reaction derived after incubation of the protein L agarose beads with the lysate, Lane 3: 15 μl of Fab 65 immobilized on protein L agarose. Numbers 1–10 on lane 1 indicate the excised protein bands used for mass spectrometry. Spot number 10 was considered as a single band. Arrows indicate the 17, 22, 25, 32, 46, 58, 80, 100 and 135 kDa molecular mass standard positions