Fig. 1

Production and screening of TLR4-overexpressing sheep. a The TLR4 expression vector. Ovine TLR4 CDS was inserted into the p3S-LoxP vector, which possessed the promoter CMV and SV40 polyA. The probe for Southern blot was used to screen the TLR4-overexpressing sheep. b The Southern blot analysis for the DNA sample from the TLR4 expression vector and the ovine ear tissue. Lane 1 is 5 × sample of the TLR4 expression vector, Lane 2 is a 1 × sample of the TLR4 expression vector, Lanes 3–6 are WT individuals, and Lane 7 is a Tg (TLR4-overexpressing) individual. c The mRNA expression of TLR4 in peripheral blood mononuclear cells quantified with real-time PCR