Fig. 2

Effects of circ_0000396 on the growth and inflammation of RASFs. RASFs were transfected with pcNDA, circ_0000396, si-NC, or si-circ_0000396. After transfection, a circ_0000396 expression was detected by qRT-PCR (Student’s t test); b, c the proliferation of RASFs was analyzed using colony formation assay and CCK-8 assay, respectively (Mann–Whitney U-test); d the number of apoptotic RASFs was examined by flow cytometry (Student’s t test); e western bolt analysis of Ki67, cleaved caspase3 and cleaved PARP expression in RASFs was performed (Student’s t test); f–I the level of inflammatory cytokines IL-6, IL-1β, IL-8 and TNF-α in RASFs was measured using ELISA assay (Student’s t test). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001