Fig. 2
Assessment of the effects of the epigenetic probes on MEL cell proliferation in culture. a Depiction of the protocol followed for the determination of the IC50 concentration of the probes. b Table showing each compound’s target as well as the identified IC50 concentration. c Time-dependent kinetic analysis of the effect of the 12 compounds on the ability of MEL cells to proliferate in culture. Cells were treated continuously with each of the compounds shown in the individual diagrams at their corresponding IC50 concentration for each probe, and the total cell number was determined daily by cell counting via a Neubauer chamber. All but A-366 compounds showed a mild to severe inhibition of cell proliferation in culture