Skip to main content
Fig. 3 | Journal of Biological Research-Thessaloniki

Fig. 3

From: The histone methyltransferase inhibitor A-366 enhances hemoglobin expression in erythroleukemia cells upon co‐exposure with chemical inducers in culture

Fig. 3

Assessment of the effect of the 12 methyltransferase inhibitors on the MEL cell erythroid differentiation program. a MEL cell growth determined after 48 h of treatment with the indicated compounds. For this experiment, all probes were co-administered with the inducer of differentiation, HMBA (5 × 10− 3 M; 0.5 mM) and cell growth was determined by cell counting (in a Neubauer chamber). b MEL cell differentiation determined after 48 h and/or 72 h of treatment with the indicated compounds at a concentration equal to their IC50 (shown in Fig. 2b). For this experiment, all epigenetic probes were co-administered with the inducer of differentiation, HMBA. Cell differentiation was determined by staining with benzidine and scoring benzidine-positive cells in culture. At least 250 cells were scored as positive or negative in each technical replication, and three technical replications were performed for each sample. **p < 0.005; *p < 0.01; Student’s t-test. c Table summarizing the information of a and b. Each experiment was conducted in at least three biological replications. Data in all panels represent means ± standard deviation

Back to article page