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Table 1 Primers used in this study for plasmids construction

From: Metabolic engineering of Escherichia coli for the utilization of ethanol

PrimersSequences
EcadhE_F_BamHICGCGGATCCGATGGCTGTTACTAATGTCGCTGAAC
EcadhE_R_XhoICCGCTCGAGTTAAGCGGATTTTTTCGCTTTTTTCTC
EcadhP_F_NcoICATGCCATGGATATGAAGGCTGCAGTTGTTACGAAGG
EcadhP_R_XhoICCGCTCGAGTTAGTGACGGAAATCAATCACCATG
EcaldA_F_NcoICATGCCATGGGCATGTCAGTACCCGTTCAAC
EcaldA_R_XhoICCGCTCGAGTTAAGACTGTAAATAAACCACCTGG
TrcEcaldA_F_XhoICCGCTCGAGCTGTTGACAATTAATCATCCGGC
TrcEcaldA_R_EcoRICCGGAATTCTTAAGACTGTAAATAAACCACCTGG
AnalcA_F_NcoICATGCCATGGATATGTGCATCCCGACCATGCAGTG
AnalcA_R_XhoICCGCTCGAGTTATTCCGGCATTTCCAGAACG
AnaldA_F_NcoICATGCCATGGATATGTCTGACCTGTTCACCACC
AnaldA_R_XhoICCGCTCGAGTTAAGCGAACAGAGCGTCACC
TrcAnaldA_F_PstIAAAACTGCAGCTGTTGACAATTAATCATCCGGC
TrcAnaldA_R_SalIACGCGTCGACTTAAGCGAACAGAGCGTCACC
Ecacs_F_NcoICATGCCATGGGCATGAGCCAAATTCACAAACACACC
Ecacs_R_BamHICGCGGATCCTTACGATGGCATCGCGATAGCCTG
  1. The restriction sites in the primers were underlined