Table 1 Primers used in this study for plasmids construction
From: Metabolic engineering of Escherichia coli for the utilization of ethanol
Primers | Sequences |
|---|---|
EcadhE_F_BamHI | CGCGGATCCGATGGCTGTTACTAATGTCGCTGAAC |
EcadhE_R_XhoI | CCGCTCGAGTTAAGCGGATTTTTTCGCTTTTTTCTC |
EcadhP_F_NcoI | CATGCCATGGATATGAAGGCTGCAGTTGTTACGAAGG |
EcadhP_R_XhoI | CCGCTCGAGTTAGTGACGGAAATCAATCACCATG |
EcaldA_F_NcoI | CATGCCATGGGCATGTCAGTACCCGTTCAAC |
EcaldA_R_XhoI | CCGCTCGAGTTAAGACTGTAAATAAACCACCTGG |
TrcEcaldA_F_XhoI | CCGCTCGAGCTGTTGACAATTAATCATCCGGC |
TrcEcaldA_R_EcoRI | CCGGAATTCTTAAGACTGTAAATAAACCACCTGG |
AnalcA_F_NcoI | CATGCCATGGATATGTGCATCCCGACCATGCAGTG |
AnalcA_R_XhoI | CCGCTCGAGTTATTCCGGCATTTCCAGAACG |
AnaldA_F_NcoI | CATGCCATGGATATGTCTGACCTGTTCACCACC |
AnaldA_R_XhoI | CCGCTCGAGTTAAGCGAACAGAGCGTCACC |
TrcAnaldA_F_PstI | AAAACTGCAGCTGTTGACAATTAATCATCCGGC |
TrcAnaldA_R_SalI | ACGCGTCGACTTAAGCGAACAGAGCGTCACC |
Ecacs_F_NcoI | CATGCCATGGGCATGAGCCAAATTCACAAACACACC |
Ecacs_R_BamHI | CGCGGATCCTTACGATGGCATCGCGATAGCCTG |