Table 2 Strains and plasmids used in this study
From: Metabolic engineering of Escherichia coli for the utilization of ethanol
| Strains or plasmids | Genotype/Description | Sources |
|---|---|---|
| Strains | ||
| E. coli DH5α | F − φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK −, mK+) phoA supE44 λ − thi-1 gyrA96 relA1 | Transgen Biotech |
| E. coli Top10 | F − mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ − rpsL(StrR) endA1 nupG | Transgen Biotech |
| E. coli JM109 | F − traD36 proA+B+ lacIq Δ(lacZ)M15/Δ(lac-proAB) glnV44 e14 − gyrA96 recA1 relA1 endA1 thi hsdR17 | Transgen Biotech |
| E. coli BL21(DE3) | F − ompT hsdSB (r−B m−B) gal dcm rne131 (DE3) | Transgen Biotech |
| Plasmids | ||
| pUC57 | Ampr oripUC | BGI |
| pTrcHis2B | Ampr oripBR322 lacIq Trcp | Invitrogen |
| pACYCDuet-1 | Cmr orip15A lacIq T7p | Novagen |
| pTrc-EcadhE | pTrcHis2B harboring E. coli adhE gene | This study |
| pTrc-EcadhP | pTrcHis2B harboring E. coli adhP gene | This study |
| pTrc-EcaldA | pTrcHis2B harboring E. coli aldA gene | This study |
| pTrc-EcadhEaldA | pTrcHis2B harboring E. coli adhE and aldA gene | This study |
| pTrc-EcadhPaldA | pTrcHis2B harboring E. coli adhP and aldA gene | This study |
| pUC-AnalcA | pUC57 harboring A. nidulans alcA gene | This study |
| pUC-AnaldA | pUC57 harboring A. nidulans aldA gene | This study |
| pTrc-AnalcA | pTrcHis2B harboring A. nidulans alcA gene | This study |
| pTrc-AnaldA | pTrcHis2B harboring A. nidulans aldA gene | This study |
| pTrc-AnalcdA | pTrcHis2B harboring A. nidulans alcA and aldA genes | This study |
| pA-Ecacs | pACYCDuet-1 harboring E. coli acs gene | This study |
| pA-EfmvaES | pACYCDuet-1 harboring E. faecalis mvaE and mvaS genes | [39] |