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Table 2 Strains and plasmids used in this study

From: Metabolic engineering of Escherichia coli for the utilization of ethanol

Strains or plasmidsGenotype/DescriptionSources
Strains
 E. coli DH5αF  φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK , mK+) phoA supE44 λ  thi-1 gyrA96 relA1Transgen Biotech
 E. coli Top10F  mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ  rpsL(StrR) endA1 nupGTransgen Biotech
 E. coli JM109F  traD36 proA+B+ lacIq Δ(lacZ)M15/Δ(lac-proAB) glnV44 e14  gyrA96 recA1 relA1 endA1 thi hsdR17Transgen Biotech
 E. coli BL21(DE3)F  ompT hsdSB (rB mB) gal dcm rne131 (DE3)Transgen Biotech
Plasmids
 pUC57Ampr oripUCBGI
 pTrcHis2BAmpr oripBR322 lacIq TrcpInvitrogen
 pACYCDuet-1Cmr orip15A lacIq T7pNovagen
 pTrc-EcadhEpTrcHis2B harboring E. coli adhE geneThis study
 pTrc-EcadhPpTrcHis2B harboring E. coli adhP geneThis study
 pTrc-EcaldApTrcHis2B harboring E. coli aldA geneThis study
 pTrc-EcadhEaldApTrcHis2B harboring E. coli adhE and aldA geneThis study
 pTrc-EcadhPaldApTrcHis2B harboring E. coli adhP and aldA geneThis study
 pUC-AnalcApUC57 harboring A. nidulans alcA geneThis study
 pUC-AnaldApUC57 harboring A. nidulans aldA geneThis study
 pTrc-AnalcApTrcHis2B harboring A. nidulans alcA geneThis study
 pTrc-AnaldApTrcHis2B harboring A. nidulans aldA geneThis study
 pTrc-AnalcdApTrcHis2B harboring A. nidulans alcA and aldA genesThis study
 pA-EcacspACYCDuet-1 harboring E. coli acs geneThis study
 pA-EfmvaESpACYCDuet-1 harboring E. faecalis mvaE and mvaS genes[39]