Fig. 1

The expression of circCTNNA1 and CXCL5 in CRC tumor tissues and cells. a The diagram depicting the structure of the CTNNA1 gene and the structure of the circCTNNA1. b The expression of circCTNNA1 in CRC tumor tissues (Tumor) and adjacent normal tissues (Normal) was determined by qRT-PCR. c QRT-PCR was employed to detect the circCTNNA1 expression in NCM460 cells and CRC cell lines (CACO2, LOVO, HT29, SW620 and SW480). d RNase R assay was used to evaluate the stability of circCTNNA1 in SW620 and SW480 cells. e Subcellular localization assay was performed to confirm the distribution of circCTNNA1 in the nuclear and cytoplasm of SW620 and SW480 cells. f, g The mRNA and protein expression levels of CXCL5 in CRC tumor tissues (Tumor) and adjacent normal tissues (Normal) were examined using qRT-PCR and WB analysis. h WB analysis was used to test CXCL5 protein expression in NCM460 cells and both CRC cell lines (SW620 and SW480). i Pearson coefficient correlation was used to analyze the correlation between circCTNNA1 and CXCL5. **p < 0.01, ***p < 0.001